Superfi Ii Master Mix (2024)

1. Platinum™ SuperFi II PCR Master Mix - Thermo Fisher Scientific

  • Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, ...

  • Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

2. [PDF] Invitrogen Platinum SuperFi II PCR Master Mix - Thermo Fisher Scientific

  • 3 mei 2022 · ideal for cloning, mutagenesis, and other applications. ∤Platinum™ SuperFi™ II PCR Master Mix is a ready-to-use ... Platinum™ SuperFi™ II PCR ...

3. Invitrogen™ Platinum™ SuperFi II PCR Master Mix - Fisher Scientific

  • Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling ...

  • Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

4. Invitrogen™ Platinum™ SuperFi II DNA Polymerase Enzyme, PCR ...

  • Platinum SuperFi II Green PCR Master Mix offers the convenience of direct gel loading of PCR products, eliminating tedious steps of dye addition to PCR samples ...

  • Thermo Fisher Scientific™ Invitrogen™ Platinum™ SuperFi II DNA Polymerase (DNA Polymerase) is a hot-start, engineered proofreading DNA polymerase that provides superior fidelity and originality to your PCR. With >300x Taq fidelity and a specially formulated buffer for primer annealing at 60°C, Thermo Fisher Scientific Invitrogen™ Platinum™ SuperFi II DNA Polymerase offers efficiency and simplicity in PCR applications that require the highest PCR accuracy such as cloning, sequencing and mutagenesis. Applications High throughput and fidelity PCR Cloning and sub-cloning Site-directed mutagenesis Amplification of GC-rich templates Template generation for sequencing Amplification of samples with suboptimal purity Long PCR Fast PCR

5. Platinum™ SuperFi II PCR Master Mix Invitrogen™, 12368010 (Price on re

  • IRIGHT aims to find the best procurement solutions for customers worldwide while promoting the finest products from China to the global market. Service. Service ...

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6. Platinum™ SuperFi™ II PCR Master Mix

7. Platinum™ SuperFi II PCR Master Mix - BMB Core Supply Center

  • Platinum™ SuperFi II PCR Master Mix. $342.00. Platinum™ SuperFi II PCR Master Mix quantity. Add to cart. SKU: 12368010 Category: Life Technologies (Thermo ...

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8. PLATINUM SUPERFI II MASTER MIX 100 RXN Thermo Fisher - BMLabosis

  • PLATINUM SUPERFI II MASTER MIX 100 RXN Thermo Fisher. PLATINUM SUPERFI II MASTER MIX 100 RXN Thermo Fisher. Marka:Diğer Ürünler. 28.215,00 TL. Ürün, Ölçü, Fiyat ...

  • Custom Products: BM Lab. Hizmetleri DNA Sequencing Analysis Monoclonal & Polyclonal Anticor Özel Primer – Prob Synthesis

9. 1SET PLATINUM SUPERFI II PCR MASTER MIX (100RXN) - Yorlab

  • 1SET PLATINUM SUPERFI II PCR MASTER MIX (100RXN). From £265.00 per (ex VAT). 1SET PLATINUM SUPERFI II PCR MASTER MIX (100RXN). Product Code, 16445389. Unit of ...

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10. 12368010 - Integrated Gulf Biosystems

  • 12368010. PLATINUM SUPERFI II MASTER MIX. 12368010. $1.00. None. 12368010 quantity. Add to cart. Categories: Molecular Biology Consumables, Offers, ...

  • PLATINUM SUPERFI II MASTER MIX

11. Invitrogen Platinum™ SuperFi™ II DNA Polymerase

  • Reduced pipetting—Master mix options available with or without direct gel-loading dyes. Find out more at thermofisher.com/platinumsuperfi. Read more. About ...

  • Invitrogen™ Platinum™ SuperFi II DNA Polymerase is a hot-start, engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer specially formulated for primer annealing at 60°C, Platinum SuperFi II DNA Polymerase offers efficiency and simplicity in PCR applications requiring highest PCR accuracy, such as cloning, sequencing, and mutagenesis.

12. Early PCR and clean-up - Oxford Nanopore Support

  • We strongly recommend the use of 2X Platinum™ SuperFi™ II Green PCR Master Mix as we have found this high fidelity polymerase performs well for UMI PCRs. The ...

  • - This protocol uses gDNA - Unique molecular identifiers (UMIs) tag the target strand copies of gDNA - Select the genomic target of your choice - Library preparation time ~6 hours - Includes PCR and fragmentation
    For Research Use Only __This is a Legacy product__ This kit is soon to be discontinued and we recommend all customers to upgrade to the latest chemistry for their relevant kit which is available on the Store. If customers require further support for any ongoing critical experiments using a Legacy product, please contact Customer Support via email: support@nanoporetech.com.

13. [PDF] Platinum SuperFi II PCR Master Mix

  • 5 sep 2019 · ∤The annealing temperature with Platinum™ SuperFi™ II. DNA Polymerase is 60°C. Proprietary additives in the.

14. Platinum Superfi (Thermo Fisher) | Ratings For Life-Science Research

  • platinum superfi ii pcr master mix. Platinum Superfi Ii Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100 ...

  • Search for Bioz rated products from peer reviewed research papers in life science.

15. [PDF] Land at PCR success - Fisher Scientific

  • Platinum SuperFi II PCR Master Mix. 100 reactions. 12368010. 500 reactions. 12368050. 2,500 reactions. 12368250. Platinum SuperFi II Green PCR Master Mix. 100 ...

16. Platinum™ SuperFi II PCR Master Mix (Invitrogen™) - Ovamedi

  • Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for ...

  • Ovamedi - Platinum™ SuperFi II PCR Master Mix

17. PLATINUM SUPERFI II MASTER MIX 500 RXN,12368050,Applied ...

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18. Invitrogen Platinum SuperFi II PCR Master Mix - IndiaMART

  • Get Invitrogen Platinum SuperFi II PCR Master Mix in Chennai, Tamil Nadu at best price by Saaskin Corporation Private Limited. Also find RT PCR Test Kit ...

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19. PLATINUM SUPERFI II MASTER MIX 100 RXN - 佑研匠簇网上商城

  • PLATINUM SUPERFI II MASTER MIX 100 RXN. PLATINUM SUPERFI II MASTER MIX 100 RXN. 产品编号:12368010 品牌:invitrogen 原厂货号:12368010; 产品分类:; 应用分类 ...

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20. Platinum superfi ii green pcr master mix-500 réactions - ugap.fr

  • Platinum superfi ii green pcr master mix-500 réactions ... Franco de port et d'emballage pour toutes les commandes supérieures à 80€ HT. Produit ni repris ni ...

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21. Invitrogen™ Platinum™ SuperFi II DNA Polimeraz Enzimi, PCR ...

  • Invitrogen™ Platinum™ SuperFi II DNA Polimeraz Enzimi, PCR Master Mix, Green PCR Master Mix Kitleri. Thermo Fisher Scientific™ Invitrogen™ Platinum™ SuperFi II ...

  • Thermo Fisher Scientific™ Invitrogen™ Platinum™ SuperFi II DNA Polimeraz (DNA Polymerase), PCR'nize üstün aslına uygunluk ve özgünlük sağlayan, sıcak başlangıçlı, mühendislik ürünü bir düzeltme okuma DNA polimerazıdır. >300x Taq aslına uygunluğu ve 60°C'de primer tavlama için özel olarak formüle edilmiş tamponu ile Thermo Fisher Scientific Invitrogen™ Platinum™ SuperFi II DNA Polimeraz, klonlama, dizileme ve mutajenez gibi en yüksek PCR doğruluğu gerektiren PCR uygulamalarında verimlilik ve basitlik sunar. Uygulamalar Yüksek kaliteli ve verimli PCR Klonlama ve alt klonlama Bölgeye yönelik mutagenez GC açısından zengin template amplifikasyonu Sıralama için şablon oluşturma Optimum olmayan saflıkta numunelerin amplifikasyonu Uzun PCR Hızlı PCR

Superfi Ii Master Mix (2024)

FAQs

What does 2x master mix mean? ›

2x PCR master mix means that it is 2 fold concentrated. As in the previous answers mentioned, you do not need to dilute the master mix prior use. You can directly set up your PCR reaction. You only need to keep in mind that half of the final volume of your reaction has to be your PCR master mix.

What does PCR Master Mix contain? ›

PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

How to do a master mix? ›

How to Master a Song
  1. Finish your mix.
  2. Bounce down your stereo track.
  3. Start a new session and Import your stereo mix and reference tracks.
  4. Listen to the mix and take notes.
  5. Limit the mix.
  6. Make level corrections with Master Rebalance if necessary.
  7. Add clarity to the low end.
  8. Correct the tone with EQ.
Sep 8, 2023

Can you vortex PCR master mix? ›

Do not vortex PCR mix. Add DNA polymerase (Taq) to the reaction tube last. Adjust electrophoresis voltage and run time to improve band resolution.

How long can you keep PCR master mix? ›

PCR Master Mix is stable for 3 months when stored at 4°C. Do you need a particular configuration for your master mix? Learn more about custom options for this product at www.promega.com/custom/. Also contact us for information on bulk purchases.

Why do we need to add buffer into the master mix? ›

The buffer keeps the pH of the reaction mixture stable. When the enzyme is added, the reaction starts, and the enzyme is therefore not included in the master mix.

What is 5x master mix? ›

Taq 5XMaster Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is best for routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA.

What is the advantage of master mix? ›

Using a master mix reduces pipetting and risk of contamination, is convenient, saves time and preempts possible errors in mixing, making it ideal for high-throughput applications.

What is the best master mix for multiplex PCR? ›

SuperPlex Premix is ideal for multiplex PCR, with unbiased amplification of different targets and high amplicon yield.

What dye is in PCR master mix? ›

2× PCR Master Mix (with Dye) is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water, in which bromophenol blue dye is included.

Why is it important to prepare a PCR master mix? ›

A master mix is a mixture containing the precursors and enzymes necessary to run a PCR assay. Using a PCR master mix for real-time PCR assays offers several advantages. A master mix enables faster setup with less pipetting as the mix can be prepared at one time and divided among multiple pipettes to save time.

How long does it take to master a mix? ›

Mastering time depends greatly on the quality of the mix. Usually, it takes a professional audio engineer 30-90 minutes for an average track. However, the time can vary from 10 minutes for a perfect mix to several hours in case of stem mastering.

What should my mix be before mastering? ›

Ideally, your mix should have a dynamic range of around 6-8 dB. This range allows the mastering engineer to make enhancements without encountering issues like distortion or excessive compression.

Can you master your own mix? ›

If your mix is good sounding and balanced, you shouldn't have to do much else to it. Just get the volume up to the commercial level. In this case it's totally fine to master the song in the mixing phase. Just put a limiter at the end of your mix bus (master fader) as a final plugin.

Why is it helpful to prepare a master mix when setting up PCR reactions? ›

With a PCR master mix, you can get a faster setup with fewer pipettes. Once a mix is prepared, it can be divided among pipettes to save time.

How to prepare dNTP mix for PCR? ›

If you increase the dNTP concentration, you must also increase the Mg2+ concentration. For best results, prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each). For example, for the preparation of 100 μl nucleotide mix, add 10 μl of dATP, dCTP, dGTP, and dTTP to 60 μl Water, PCR Grade*.

What does the buffer do in PCR master mix? ›

Typically, a buffer is a solution that can resist pH changes by chemically neutralizing small amounts of added acidic or basic compounds, thus maintaining the overall pH of a medium. Why is this necessary for PCR? DNA is pH-sensitive.

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